With an ever-increasing need of skilled healthcare workers, efficient learning methods like Virtual Reality are becoming increasingly important. A VR simulation for endotracheal suction intervention was developed and tested. The aim of this pilot study is to examine the VR simulation’s acceptance and knowledge improvement. Furthermore, the effect of presence on acceptance and knowledge improvement was investigated. A total of 51 students participated in the pilot study, using pre-post-test design. A modified Unified Theory of Acceptance and Use of Technology (UTAUT) and Igroup Presence Questionnaire (IPQ) were used. Correlation and regression analysis were performed. Pre- and post-tests showed a significant knowledge improvement (p<0.001). Correlation between presence and behavioural intention was highly positive (r=0.52, p<0.001). Performance and effort expectancy are dominant in effect on behavioural intention of using VR learning simulation. The results indicate that a simulation, which conveys a higher sense of presence, is more likely to be accepted.